MoBiTec GmbH is Offering SURE Gene Expression System for Bacillus subtilis, Regulated by Subtilin

A system for SUbtilin-Regulated gene Expression (SURE) in Bacillus subtilis is offered. Subtilin is a small peptide antibiotic produced by Bacillus subtilis. The SURE system provides an alternative to, e.g., IPTG or xylose inducible systems.

Conducted by the Dutch company NIZO food research BV, the SURE system, a SUbtilin-Regulated Expression system for Bacillus subtilis was developed which allows strict control of gene expression by addition of subtilin.

Subtilin is a small peptide antibiotic of 32 amino acids produced by Bacillus subtilis. Subtilin production and regulation is encoded in the chromosome by a cluster of 9 genes that are transcribed from two promoters. Subtilin regulates/activates its own biosynthesis via a two component regulatory system.

For the SURE system the regulatory components called SpaR (response regulator) and SpaK (membrane sensor) were isolated and placed on the chromosome of a B. subtilis host strain. One of the subtilin-regulated promoters is placed on a plasmid followed by a multiple cloning site. Upon addition of subtilin the system is activated and a protein can be produced either intracellularly or secreted into the the growth medium.

Advantages of SURE:

- Tightly controlled gene expression in a bacterium with long biotechnology history
- Genome sequence available
- Longstanding genetic engineering experience
- Complete set of genetic engineering tools is available
- Potentially useful for cloning of genes with toxic products
- Can be used for the identification of essential genes after insertion into the chromosome in front of the gene in question
- Potential for secretion of gene products
- Controlled gene expression for metabolic engineering

MoBiTec GmbH, Germany, is offering the SURE system to academic customers worldwide.

MoBiTec GmbH is offering superior research tools for molecular and cell biology. In parallel to its own product line,
MoBiTec distributes products from several international companies in Germany and across the world.

MoBiTec GmbH
Lotzestr. 22a
Arne Schulz
37083 Goettingen

This release was published on openPR.