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Transcreener® GDP GTPase Assay - The Only Mix-and-Read Fluorescent GTPase Assay, by BellBrook Labs

09-18-2018 01:19 PM CET | Science & Education

Press release from: MoBiTec GmbH

GDP-Assay-Schematic

GDP-Assay-Schematic

The Transcreener GDP GTPase assay is a homogenous, fluorescent immunoassay that monitors the activity of GTPases along with GEF and GAP partners. The assay directly measures the amount of GDP produced in an in vitro GTPase reaction.

BellBrook Labs' Transcreener GDP GTPase assays are available with fluorescence polarization (FP), fluorescence intensity (FI), and far-red, time-resolved Forster-resonance-energy-transfer (TR-FRET) readouts that provide a safe, HTS-compatible alternative to cumbersome radioassay methods and are more sensitive and less subject to interference than colorimetric phosphate detection methods.

Based on BellBrook Labs' proprietary nucleotide immunodetection technology, the Transcreener® GDP GTPase Assays are compatible with any enzyme class that produces GDP, including monomeric small G proteins, such as CDC42, and Gα subunits of heterotrimeric G proteins, such as Gαi1.
GDP displaces tracer from a highly selective monoclonal antibody resulting in a decrease in fluorescence polarization or an increase in fluorescence intensity.

• Measure guanine nucleotide exchange factor (GEF) activity
• Measure GTPase-activating proteins (GAP) activity
• Homogenous, simple, mix-and-read assay
• Tunable dynamic range to match the target of interest
• Detect any GDP producing enzyme: GTPases, fucosyltransferases, mannosyltransferases

Three Readouts Available

• Fluorescence polarization (FP)
• Fluorescence intensity (FI)
• Time-resolved Forster-resonance-energy-transfer (TR-FRET)

Assay Features

• Easy-to-Use, Ultra-Sensitive GTPase Activity Assay
• HTS Compatible with 96, 384, and 1536-well plates
• Direct detection of GDP to monitor GTPase, GAP, and GEF Activity
• Stable assay reagents – minimum 8-hour reagent and signal stability

Far Red FP, FI &TR-FRET Readouts Validated on Major Multimode Readers

Model

Tecan
Infinite® M200, Infinite® F200/Ultra evolution, Infinite® F500, Infinite® M1000/M1000Pro/ Safire2™, Spark™ 10M

PerkinElmer
EnVision®/EnVision® Xcite

Molecular Devices
Analyst® GT/ HT, Gemini® XPS/EM, Spectra-Max® M2/M2e, SpectraMax® M5/M5e/ FlexStation® 3, SpectraMax® Paradigm

BMG Labtech
CLARIOstar®, FLUOstar® Omega, PHERAstar® FSX, PHERAstar® Plus/FS, POLARstar® Omega

BioTek
Cytation™ 5/3/1, Synergy™ H1, Synergy™ 2/H4 /4, Synergy™ HTX, Synergy™ Neo 2

Berthold
TriStar²S LB 942, Mithras² LB 943

All BellBrook Labs products can be ordered in Europe from MoBiTec: http://www.mobitec.com

Contact info@mobitec.com for more information and bulk pricing options.

About Bellbrook Labs

BellBrook Labs is dedicated to providing the pharmaceutical industry with enabling screening tools to accelerate the discovery of more effective therapies. As a small, R&D-driven company, BBL identifies areas where there is a strong need but a significant technical hurdle, and then BBL develops a solution. The company is focused on taking creative approaches to new product development and to continually improving the level of service provided to our customers. The company has built its reputation on Transcreener®, a biochemical assay platform. www.bellbrooklabs.com

About MoBiTec GmbH

MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.

In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.

MoBiTec GmbH, Lotzestr. 22a, 37083 Goettingen,
Germany, info@mobitec.com, www.mobitec.com

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