Real-time PCR Thermal Cyclers Research:CAGR-6 of 6.1% in the next six years

QY Research Inc. (Global Market Report Research Publisher) announces the release of 2025 latest report "Real-time PCR Thermal Cyclers- Global Market Share and Ranking, Overall Sales and Demand Forecast 2026-2032". Based on current situation and impact historical analysis (2020-2024) and forecast calculations (2026-2032), this report provides a comprehensive analysis of the global Real-time PCR Thermal Cyclers market, including market size, share, demand, industry development status, and forecasts for the next few years.

The global market for Real-time PCR Thermal Cyclers was estimated to be worth US$ 3198 million in 2024 and is forecast to a readjusted size of US$ 5031 million by 2031 with a CAGR of 6.8% during the forecast period 2025-2031.

【Get a free sample PDF of this report (Including Full TOC, List of Tables & Figures, Chart)】
https://www.qyresearch.com/reports/4588574/real-time-pcr-thermal-cyclers

Real-time PCR Thermal Cyclers Market Overview

Product Definition

Real-time PCR thermal cyclers are core equipment in the field of molecular biology for nucleic acid amplification and real-time detection. They integrate a fluorescence detection system into the traditional PCR thermal cycler, enabling real-time monitoring of fluorescence signal changes during nucleic acid amplification. This allows for qualitative and quantitative analysis of template nucleic acids. Compared to traditional PCR instruments that can only determine results through gel electrophoresis after amplification, real-time PCR thermal cyclers significantly shorten the experimental cycle and provide more accurate and intuitive detection data, playing an irreplaceable role in fields such as gene research, disease diagnosis, and food safety testing.

Product Image of a Tailing Dewatering Screen

Real-time PCR Thermal Cyclers

Structure and Types

To achieve precise temperature control and efficient fluorescence detection, real-time PCR thermal cyclers typically consist of multiple core components working together. The heating and cooling module is crucial for ensuring accurate temperature cycling, often employing Peltier semiconductor elements for rapid temperature rise and fall with a control accuracy of ±0.1°C, ensuring uniform temperature across all reaction wells and preventing variations in amplification efficiency due to temperature differences. The optical detection system includes an excitation source, filters, and a photodetector. The excitation source emits light of a specific wavelength to excite fluorescent substances, the filters screen for target fluorescence wavelengths, and the photodetector converts the fluorescence signal into an electrical signal, which is then transmitted to the data processing system. The sample reaction module is usually designed in 96-well or 384-well plate formats, allowing for simultaneous processing of multiple samples and improving experimental efficiency. Some devices also support micro-reaction tubes to meet the needs of detecting small-volume samples. Furthermore, the device is equipped with an embedded computer and dedicated software to control the temperature cycling program, acquire and analyze fluorescence data. The software automatically generates amplification curves, melting curves, and other graphs, and provides data export and report generation functions for convenient subsequent analysis by researchers.

Depending on the testing needs and application scenarios, real-time PCR thermal cyclers have evolved into various types. Based on the number of fluorescence detection channels, they can be categorized into single-channel, dual-channel, and multi-channel (4-6 channels) instruments. Single-channel instruments are suitable for detecting a single fluorescent label, such as qualitative experiments that only need to determine the presence of a target gene. Multi-channel instruments can simultaneously detect multiple fluorescence signals, enabling the detection of multiple target genes in the same reaction system. For example, they can simultaneously screen for multiple viruses in pathogen detection, or simultaneously detect the target gene and internal reference gene in gene expression analysis, significantly improving experimental efficiency. Based on sample processing scale, they can be categorized into conventional (96-well plates) and high-throughput (384-well plates). High-throughput instruments are more suitable for large-scale sample testing, such as batch sample screening in clinical diagnosis or high-throughput genotyping experiments in scientific research. In addition, there are specialized instruments for specific applications, such as portable real-time PCR thermal cyclers, which are compact and portable, suitable for on-site testing, such as rapid pathogen screening during public health emergencies or microbial detection in the field.

Working Principle

Its operation revolves around the core steps of PCR technology: temperature cycling of denaturation, annealing, and extension, combined with real-time acquisition and analysis of fluorescence signals. In the denaturation stage, the equipment rapidly raises the reaction system temperature to 90-95°C, causing the template DNA double strand to unwind into single strands. In the annealing stage, the temperature drops to 50-65°C, allowing specific primers to bind to complementary regions of the single-stranded template. Subsequently, in the extension stage, the temperature is adjusted to approximately 72°C, and DNA polymerase, starting from the primers, uses dNTPs in the reaction system to synthesize new DNA strands, completing one amplification cycle. During this process, fluorescent dyes or probes in the reaction system emit fluorescence as DNA amplifies. The equipment's optical detection system continuously acquires the fluorescence signal for each cycle and converts the signal into a data curve. By analyzing parameters such as the initial cycle number (Ct value) of the curve, the initial concentration of the template nucleic acid or the presence of the target nucleic acid can be determined.

Application

Real-time PCR thermal cyclers have a wide range of applications across multiple fields. In medical diagnostics, they can be used for pathogen detection, such as rapid qualitative and quantitative detection of COVID-19, influenza virus, and hepatitis B virus, helping clinicians to promptly assess patient infection status and develop treatment plans. They can also be used for tumor marker detection, providing a basis for early tumor diagnosis and prognostic assessment by analyzing the mutation or expression levels of tumor-related genes. In life science research, it is an important tool for gene expression analysis, comparing the expression differences of target genes in different tissues or under different treatment conditions. It can also be used for gene cloning, gene mutation detection, SNP genotyping, and other experiments, providing support for gene function research and the exploration of genetic disease mechanisms. In food safety, it can rapidly detect pathogenic bacteria (such as Salmonella and E. coli), genetically modified components, or veterinary drug residues in food, ensuring food quality and safety. In agriculture, it can be used for early detection of crop diseases and pests, such as screening for plant viral diseases or diagnosing livestock and poultry diseases, contributing to the healthy development of agricultural production.

Examples of Important Parameters

Parameter Name

Meaning Explanation

Typical Range/Description

Temperature Control Accuracy

Deviation between actual and set temperatures

±0.1°C~±0.3°C

Temperature Uniformity

Temperature consistency between wells

±0.2°C~±0.4°C (lower is better)

Temperature Rise/Fall Rate

Heating and cooling rates

2.5~6°C/s (high-end models can reach 8°C/s)

Cyclic Repeatability

Temperature stability and data consistency after multiple cycles

Ct value repeatability SD < 0.2

Number of Fluorescence Detection Channels

Number of fluorescence signals detected simultaneously (corresponding to different dyes)

2~6 channels (high-end models can reach 8 or 10 channels)

Detection Sensitivity

Minimum detectable copy number

Single copy to 10 copy level

Dynamic Detection Range

Detectable concentration range

≥9 orders of magnitude (109 times dynamic range)

Optical System Type

Light source and detection method

LED + photomultiplier tube/CCD/CMOS array

Sample Throughput

Number of samples that can be detected at one time

16, 48, 96, 384 wells are common

Reaction Volume

Reaction volume per well

5~100 μL (commonly 10~50 μL)

Calibration Method

Optical and temperature calibration mechanisms

Automatic/semi-automatic (built-in standard curve)

Data Analysis Functions

Types of analysis that the software can perform

Quantitative analysis, melting curve, genotyping, relative quantification

Compatible Consumables

Support for general or special reaction plates/tubes

0.1 mL, 0.2 mL tubes or 96/384-well plates

Communication Interface

Data export and networking capabilities

USB, Wi-Fi, LAN, cloud transmission

Dimensions and Noise

Instrument size and operating noise

Miniature models

About Us:
QYResearch founded in California, USA in 2007, which is a leading global market research and consulting company. Our primary business include market research reports, custom reports, commissioned research, IPO consultancy, business plans, etc. With over 19 years of experience and a dedicated research team, we are well placed to provide useful information and data for your business, and we have established offices in 7 countries (include United States, Germany, Switzerland, Japan, Korea, China and India) and business partners in over 30 countries. We have provided industrial information services to more than 60,000 companies in over the world.

Contact Us:
If you have any queries regarding this report or if you would like further information, please contact us:
QY Research Inc.
Add: 17890 Castleton Street Suite 369 City of Industry CA 91748 United States
EN: https://www.qyresearch.com
Email: global@qyresearch.com
Tel: 001-626-842-1666(US)
JP: https://www.qyresearch.co.jp

This release was published on openPR.