Introducing cADDis: The Simplest Assay in the World for Detecting cAMP

Cyclic AMP (cAMP) is the prototypical second messenger. The major receptor in eukaryotic cells, cAMP-dependent Protein Kinase (PKA), is ubiquitous. cAMP affects many different physiological and biochemical processes including the activity of kinases.

cADDis - An elegant means to measure cAMP signaling in real time, in the living cells of your choice.

Save time and money by switching to cADDis, a simple and robust assay for Gs and Gi signaling, without the need for co-factors, cell lysis, or complex liquid handling. cADDis is genetically-encoded and packaged in BacMam for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add cADDis to your cells and measure robust signals the very next day on standard fluorescence microscopes and automated plate readers.

Gs detection: Kinetics. Dose responses. Bright and reliable signals.

Measure kinetics and dose responses of Gs-mediated cAMP production with large and consistent fluorescence changes. cADDis is extremely bright with remarkable signal to noise (Z' > 0.95). Examplary data available on request for: cADDis in live HEK293 cells stimulated with isoproterenol, a selective agonist of ß-adrenergic receptors.

Gi detection: Forskolin-free

cADDis detects Gi-mediated decreases in cAMP production, in real time, without the need for forskolin or IBMX. The cADDis Gi assay yields Z' values > 0.85. Forego the forskolin and measure biologically relevant Gi signaling using cADDis. Examplary data available on request for: cADDis in live HEK293 cells stimulated with quinpirole, a selective agonist of dopaminergic D2 receptors.

Discover cADDis today

MoBiTec GmbH, Germany, is a certified agent for Montana Molecular in Europe. For details see http://www.mobitec.com

About Montana Molecular

Montana Molecular develops genetically encoded fluorescent biosensors and probes for cell-based assays and live cell imaging. Montana Molecular products are used for drug discovery and to observe and measure cell signaling processes.

Montana Molecular's scientific team is focused on creating breakthrough innovations that empower both basic research scientists and drug discovery teams. The company is committed to building a sustainable biotech industry in the state of Montana, providing good jobs in a safe and healthy environment.

About MoBiTec GmbH

MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.

In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.

MoBiTec GmbH
Arne Schulz
Lotzestr. 22a, 37083 Goettingen
Germany

This release was published on openPR.