MoBiTec Offers HS-Nuclease, a Recombinant Endonuclease Encoded by the Same Gene as Benzonase®

Nucleases are nucleic acid degrading enzymes capable of cleaving the phosphodiester bonds between the nucleotide subunits of its substrate. HS-Nuclease, a broad band nuclease, is an indispensable tool to reduce the viscosity of cell lysates.

Description:

HS-Nuclease, (CAS 9025-65-4) is an ultra-pure recombinant form of Serratia marcescens extracellular endonuclease (encoded by the same gene as Benzonase®) produced in E. coli using a proprietary process. This non-specific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. HS-Nuclease is a highly purified homodimer of 27 kDa subunits that has exceptionally high specific activity and is free of protease activity. HS-Nuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.

Purity:

HS-Nuclease is purified through a proprietary process that achieves a purity of >99%.

HS-Nuclease shows no detectable protease activity.

Endotoxin Level:

< 0.1 EU / 1,000 units of HS-Nuclease as determined by the Endo-Safe LAL Assay.

Applications:

HS-Nuclease can be used to

• reduce viscosity of cell lysate and reduce back pressure of column loading
• remove nucleic acid contamination from sample preparations, reduce nucleic acid contamination of Ni column purification
• reduce smearing in SDS-PAGE when used with 10% SDS or Gel Loading Dye to make whole cell lysate
• reduce or prevent clumping of concentrated cells and thawed cells
• replaces crude DNase I in many applications

The product is available to customers worldwide. For further information check:
http://www.mobitec.com/cms/products/bio/06_dna_prot_tools/nucleases1.html

MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.

In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.

Arne Schulz
MoBiTec GmbH
Lotzestr. 22a
37083 Goettingen, Germany

This release was published on openPR.