MoBiTec Launches SpeedBlot (His) – The Fastest Western Blot Stain for His-Tagged Proteins Ever

Instant detection of poly-histidine tagged proteins on nitrocellulose membrane with SpeedBlot (His) is easy and does not require relevant hands-on time. Even 0.1 pmol detection limits can be achieved with this highly sensitive detection reagent.

Western Blot is widely used in research to separate and identify proteins. In this technique a mixture of proteins is separated based on molecular weight through gel electrophoresis. The content of the gel is then transferred to a membrane producing a band for each protein. The protein of interest is then detected by means of a specific antibody.

Poly-histidine tags are often used for affinity purification of poly-histidine-tagged recombinant proteins expressed in Escherichia coli, Bacillus megaterium, Bacillus subtilis, and other prokaryotic expression systems. A poly-histidine tag is an amino acid motif in proteins that consists of multiple histidine (His) residues, mostly located at the N- or C-terminus of the protein. In order to facilitate the detection of successfully expressed his-tagged proteins the usage of anti-poly-histidine antibodies is a prevalent technique.

However, the common protocol for the antibody-based detection of the protein of interest is a laborious and time-consuming undertaking.

As time is money and to make life easier MoBiTec introduces SpeedBlot (His), a ready-to-use reagent for the detection of poly-histidine tagged proteins in blot assays:

SpeedBlot (His) is equally suitable for Western Blot assays and Dot or Slot Blot assays. The detection reagent is made of an optimized anti-histidine antibody gold nanoparticle conjugate and is reactive against C- and N-terminal tags. The protein of interest is specifically stained in pink directly on the blot.

As Easy as Ponceau Red but as Specific as ECL Antibody!

Features

• Fastest Western ever; results in 30-90 min
• Specific staining of His-tagged proteins
• Sensitive to sub-picomol amounts of protein
• Simple incubation, no buffer exchange, etc.
• Economic, no readout equipment required
• Visually detectable result
• Quantitative and permanent signal
• Works with C-/ N-terminal tags

The product is readily available. For further details please see:

http://www.mobitec.com

Time is money! (Benjamin Franklin)

MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.
In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.

MoBiTec GmbH
Arne Schulz
Lotzestr. 22a
37083 Goettingen
Germany

This release was published on openPR.