MoBiTec GmbH Offers MagSi-NGS/PREP Kit for Magnetic Next Generation Sequencing (NGS) Library Clean-Up

Magnetic bead-based MagSi-NGS/PREP kit constitutes an efficient solution for clean-up and size selection in library preparation of NGS sequencing reactions. The simple, flexible protocol can be adjusted to any specific application and NGS platform.

The availability of fast, inexpensive, and accurate genome information is of steadily increasing importance to research and healthcare, both on the academic side and in practical directions worldwide.This demand prompted the development of Next Generation Sequencing (NGS) technologies.

The preparation of high-quality libraries at high yield is an essential first step in the next generation sequencing protocol. MagSi-NGSPREP is designed for an optimized purification of next generation library preparation through efficient clean-up of the successive enzymatic reactions (i.e., end repair, dA-tailing, adaptor ligation). MagSi-NGSPREP is used for clean-up of library targeting sequencers such as Life Technologies’ ABI SOLiD, Ion Torrent; Illumina’s HiSeq 2000, MiSeq, GAIIx; and Roche’s 454.

Magnetic-bead based MagSi-NGSPREP offers an efficient solution for clean-up and size selection in library preparation of NGS sequencing reactions. The simple and flexible protocol can be adjusted to your specific application and NGS platform. MagSi-NGSPREP can be used manually but is also easy to automate for high-throughput processing. In conclusion: a simple and quick way to consistent NGS results.

Features

High quality results

- High recovery of DNA fragments, more than 70%
- Excellent removal of enzymes, primers, oligonucleotides, polymerase, and other contaminants
- Fragment size selection adjustable between 100 and 1000 base pairs
- Guarantees consistent sequencing results

Simplify your routine

- One product for all clean-up and size selection steps in the library preparation worflow
- Simple bind – wash – elute procedure
- Protocol easily adjustable for clean-up or size selection using specfic reagent-to-sample ratios
- Manual and automated use
- Meets the requirements of common library preparation kits

Easy to automate

- Fast and robust protocol resulting in a high-throughput on automated systems
- Optimized separation performance using validated magnetic separators for 96 and 384 wells PCR plates
- Compatible with many different automated liquid handling systems (e.g., PerkinElmer®, Agilent Technologies®, Beckman Coulter®)

Check out our videos on YouTube at:

http://www.youtube.com/watch?v=a3xyL3vYRAM
http://www.youtube.com/watch?v=A_Lr7zAbaEs

For details on MagSi-NGSPREP please see: http://www.mobitec.com/cms/products/bio/02_genomics/MagSi...

MoBiTec GmbH, Germany, is a privately held company, founded in 1987, that offers research tools for molecular and cell biology. Products include fluorescence reagents, kits and imaging tools, two- & one-hybrid systems for in vivo detection of molecular interactions, non-E. coli expression systems, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, affinity chromatography products as well as general laboratory equipment. In parallel to its own product lines, MoBiTec distributes products from internationally renowned companies, e.g., AnaSpec, Echelon, MBL, MirusBio, Bachem America, Sumitomo Bakelite Co., Ltd., Cyagen, BellBrook Laboratories, and EdgeBio, in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.

Arne Schulz
MoBiTec GmbH
Lotzestr. 22a
37083 Goettingen
Germany

This release was published on openPR.