Pulsed Field Gel Electrophoresis (PFGE) Systems Market Reflect a Holistic Expansion During 2021-2031

In the recent decades, the use of pulsed-field gel electrophoresis (PFGE) in the molecular biology space has been subjected to a lot of research. Pulsed-field gel electrophoresis is a robust tool used to characterize various strains at the DNA level, to obtain useful information on genome size and to create the physical and genetic map of bacteria chromosome that are poorly understood at the genetic level. Pulsed-field gel electrophoresis is also used in isolating chromosomes in microorganisms, and in the long-range mapping of mammalian genes, and can also examine the extended and oriented configuration of huge DNA molecules in agarose gels at finite field strengths.

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Genome analysis based on pulsed-field gel electrophoresis has a wide range of applications ranging from typing bacterial strains to radiobiology to cancer research, which could bring some traction in pulsed-field gel electrophoresis market. The methods for running pulsed-field gel electrophoresis have positively changed since its invention, but the method for preparing chromosomal DNA itself is essentially unchanged, which continues to restrict pulsed-field gel electrophoresis applicability, especially when analyses require many samples.

Apart from having advantage of examining the elongated and oriented configuration of large DNA molecules in agarose gels at finite field strengths, PFGE is having various other applications also. Such as, high concordance with epidemiological relatedness, stable and reproducible DNA restriction patterns, and can be applied as a universal generic subtyping method for many different bacteria with only the choice of the restriction enzyme and electrophoresis conditions optimized for each species. Besides, PFGE is said to be more discriminating than methods such as ribotyping or multi-locus sequence typing for many bacteria. Moreover, PFGE has shown excellent ability to separate small, natural linear chromosomal DNAs ranging in size from 50-kb parasite microchromosomes to multimillion-bp yeast chromosomes. However, intact human chromosomes range in size from 50 million to 250 million bp (Mb), too large for direct PFGE separations.

Pulsed Field Gel Electrophoresis (PFGE) Market: Drivers and Restraints

Primary factors driving Pulsed Field Gel Electrophoresis (PFGE) market include growing need for safe and effective guiding technology during isolation. PulseNet investigates bacterial isolates from sick people, contaminated food, and the places where food is produced. PFGE is the said to be the gold standard fingerprinting method used within PulseNet. However, some limitations of PFGE hindering the growth of pulsed field gel electrophoresis market. Time consuming process, inability to discriminate between all unrelated isolates, variation in DNA restriction patterns, and inability to optimize separation in every part of the gel at the same time, are the main limiting factors which are restricting the growth of the market. Besides, bands of same size may not come from the same part of the chromosome, change in one restriction site can result in more than one band change, and inability to differentiate isolates to the same degree that can be achieved by whole genome sequencing (WGS) are other key restraining factors.

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Pulsed Field Gel Electrophoresis (PFGE) Market: Segmentation

Tentatively, the global Pulsed Field Gel Electrophoresis (PFGE) market has been segmented on the basis of indication, end users, and geography.

Based on Types, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
• Rotating Gel Electrophoresis (RGE)
• Field-Inversion Gel Electrophoresis (FIGE)
• Transverse-Alternating Field Gel Electrophoresis (TAFE)
• Contour-Clamped Homogeneous Electric Fields (CHEF)
• Orthogonal-Field Alternation Gel Electrophoresis (OFAGE)
• Programmable Autonomously-Controlled Electrodes (PACE)
• Pulsed-Homogeneous Orthogonal Field Gel
Electrophoresis (PHOGE)

Based on applications, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
• Separation of chromosomes
• Restriction mapping of chromosome regions
• DNA fragment purification
• Others

Based on end user, the global Pulsed Field Gel Electrophoresis (PFGE) market is segmented as:
• Laboratory
• Academia
• Private Laboratories
• Biopharmaceuticals Companies
• CMOs
• Others

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Pulsed Field Gel Electrophoresis (PFGE) Market: Key Players

The global pulsed field gel electrophoresis systems market is consolidated market and dominated by the are some leading players such as Bio-Rad Laboratories, Inc, Core Life Sciences, Inc., LABREPCO, Analytik Jena AG. Technological advancement in products and expansion of geographical reach are the major trend observed in the global pulsed field gel electrophoresis systems market.

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