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The Comet Assay: Nutrition & Health

05-24-2011 09:03 AM CET | Science & Education

Press release from: Perceptive Instruments Ltd.

Last year we highlighted research linking nutritional factors, mutagenicity and carcinogenesis. One invaluable tool for investigations into DNA damage, protection and repair is the Comet Assay (Single Cell Gel Electrophoresis).

The growing popularity & acceptance of the assay is evident by the number of peer reviewed papers in the scientific literature. Here we list a selection of recent papers with nutrition & health a key theme and all using data obtained with Perceptive Instruments’ Comet Assay software (http://www.scorecomets.com) to quantify DNA damage.

[1] Anomalous genotoxic responses induced in mouse lymphoma L5178Y cells by potassium bromate (2010) C. Priestley et al, Toxicology 267(1-3):45-53
[2] Assessment of okadaic acid effects on cytotoxicity, DNA damage and DNA repair in human cells (2010) V. Valdiglesiasa et al, Mutation Research 689(1):74-79.
[3] Beauvericin and ochratoxin A genotoxicity evaluated using the alkaline comet assay: single and combined genotoxic action (2010) M.Š. Klaric et al, Archives of Toxicology. 84(8):641-650
[4] Berberine and a Berberis lycium extract inactivate Cdc25A and induce a-tubulin acetylation that correlate with HL-60 cell cycle inhibition and apoptosis (2010) M. Khan et al, Mutation Research 683(1-2):123-130
[5] Bolus ingestion of white and green tea increases the concentration of several flavan-3-ols in plasma, but does not affect markers of oxidative stress in healthy non-smokers (2010) N. Müller et al, Molecular Nutrition & Food Research 54(11):1636-1645
[6] Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: preliminary results (2010) M. Milic et al, Acta Biochimica Polonica 57(3):339-345
[7] Differential toxicity of heterocyclic aromatic amines and their mixture in metabolically competent HepaRG cells (2010) J. Dumont et al, Toxicology & Applied Pharmacology 245(2): 256-263
[8] Does potassium sorbate induce genotoxic or mutagenic effects in lymphocytes? (2010) S. Mamur et al, Toxicology In Vitro 24(3): 790-794
[9] Effects of lycopene, synbiotic and their association on early biomarkers of rat colon carcinogenesis (2010) M.C. Dias et al, Food & Chemical Toxicology 48(3): 772-780
[10] Genistein and ß-carotene enhance the growth-inhibitory effect of trichostatin A in A549 cells (2010) R-J. Shiau et al, European Journal of Food Nutrition 49(1): 19-25
[11] Impact of paper filtered coffee on oxidative DNA-damage: Results of a clinical trial (2010) M. Mišík et al, Mutation Research 692(1-2): 42-48
[12] Increasing fish consumption does not affect genotoxicity markers in the colon in an intervention study (2010) G.K.Pot et al, Carcinogenesis 31(6): 1087-1091
[13] Influence of diet on oxidative DNA damage, uracil misincorporation and DNA repair capability (2010) R.P. Prado et al, Mutagenesis 25(5): 483-487
[14] Instant coffee with high chlorogenic acid levels protects humans against oxidative damage of macromolecules (2010) C. Hoelzl et al, Molecular Nutrition & Food Research 54(12): 1722-1733
[15] Polyphenolic Apple Extracts: Effects of Raw Material and Production Method on Antioxidant Effectiveness and Reduction of DNA Damage in Caco-2 Cells (2010) P. Bellion et al, Journal of Agricultural & Food Chemistry 58(11): 6636-6642
[16] Polyphenolic Compounds from Salvia Species Protect Cellular DNA from Oxidation and Stimulate DNA Repair in Cultured Human Cells (2010) A. A. Ramos et al, Journal of Agricultural & Food Chemistry 58(12): 7465-7471
[17] Prevention of oxidative DNA damage in inner organs and lymphocytes of rats by green tea extract (2010) N. Kager et al, European Journal of Nutrition 49(4): 227-234
[18] Evaluation of cytotoxicity and oxidative DNA damaging effects of di(2-ethylhexyl)-phthalate (DEHP) and mono(2-ethylhexyl)-phthalate (MEHP) on MA-10 Leydig cells and protection by selenium (2010) P. Erkekoglu et al, Toxicology & Applied Pharmacology 248(1): 52-62
[19] Protective effects of Ursolic acid and Luteolin against oxidative DNA damage include enhancement of DNA repair in Caco-2 cells (2010) A.A. Ramos et al, Mutation Research 692(1-2) 6-11
[20] Spectrophotometric analysis of flavonoid-DNA interactions and DNA damaging/protecting and cytotoxic potential of flavonoids in human peripheral blood lymphocytes (2010) G. Rusak et al, Chemico-Biological Interactions 188(1): 181-189
[21] Storage results in loss of the antigenotoxic properties of Lentinula edodes (Shiitake muscroom) and development of in vivo genotoxicity (2010) P.L. Alves de Lima et al, Journal o Food Biochemistry 34(1): 206-208

To view these papers (subject to access permissions) please visit our Recent Papers (http://www.scorecomets.com/assay/#papers) page.

Research scientists rely on Comet Assay IV for reproducible and accurate measurements. You can find out more about Comet Assay IV at our dedicated comet assay website (http://www.scorecomets.com).

About Perceptive Instruments

Perceptive Instruments Ltd. (www.perceptive.co.uk) develops, manufactures and supplies image analysis and data processing solutions for many areas of scientific research and industry. Our products are used primarily in the pharmaceutical & health care industries, as well as environmental monitoring, food and manufacturing industries. We have established an enviable reputation based upon our willingness and ability to completely satisfy our clients’ needs. We provide standard 'off the shelf' systems and customised solutions based on current or novel technologies. Examples of special solutions include systems for antibiotic testing, vaccine development and robot vision.

Our Products

Comet Assay IV

Comet Assay IV (www.perceptive.co.uk/cometassay/) is an interactive live video-based system for scoring cells subjected to the single cell gel electrophoresis technique (comet assay). The system is the very latest in a highly successful series of comet assay scoring systems from Perceptive Instruments that have evolved and developed since the release of our first system in 1992.

Sorcerer Colony Counter

Sorcerer Colony Counter (www.perceptive.co.uk/colony-counter/) provides instant, accurate plate counting for microbial and mammalian colonies. It is ideal for laboratories engaged in drug development and production, food and milk testing, environmental monitoring and public health.

Ames Study Manager

The Ames Study Manager (www.perceptive.co.uk/amesstudymanager/)is a study management and reporting system for Ames testing. Together with the Sorcerer automatic colony counter it provides a complete solution for regulatory and non-regulatory safety assessment.

SpeckCheck 2

SpeckCheck 2 (www.perceptive.co.uk/speckcheck2/) is the advanced scanner based system for automatic detection and analysis of dirt and other contaminants in pulp and paper.

Perceptive Instruments Ltd
Blois Meadow Business Centre
Steeple Bumpstead
Haverhill
CB9 7BN
UK
Tel. +44 (0)1440 730773
Fax. +44 (0)1440 730630
www.perceptive.co.uk
sales@perceptive.co.uk

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