The Bio-Chromatographers’ wish
DBC as a function of linear flow rate for Lysozyme on YMC-BioPro 75 µm 100 nm cation exchange material.
The data below shows the DBC measured at high flow rates. The result: up to 1000 cm/hr the DBC stays very high. With measured amounts of at least 215 mg Lysozyme per ml of resin material, it reaches levels far above traditionally used cross-linked dextran and agarose gels. The graph below confirms that this high DBC is achievable across a wide range of flow rates. The graph shows the DBC of Lysozyme on YMC-BioPro S75 which is a sulphobutyl substituted cation exchanger with a particle size of 75 µm and a pore size of 100 nm (1000 Å). With its properties it is especially well suited for capture applications. However, at the same time, it is also usable for (intermediate) purification steps.
With a pore size of 100 nm the material is particularly useful for large molecules like monoclonal antibodies. However, tests with 5 µm analytical material show that YMC-BioPro can be used across a very wide range of molecular weights. The analytical material is based on the same hydrophilic resin material as the 75 µm preparative material and therefore shows very similar properties. The DBC for anion as well as cation exchanger material has been examined. The experiments cover a complete order of magnitude from roughly 14.000 to 150.000 Da. At the same time, this versatile material shows recoveries of generally 90% and more.
As a result, YMC-BioPro can help developing very economic bio-processes. With increasing titers of fermentations, high dynamic binding capacities are needed in order to keep column sizes at usable levels. High flow rates are needed to process the material in an acceptable amount of time. As shown above YMC-BioPro aids both points.
Additionally, with the knowledge gained from almost 30 years of experience with silica based chromatography phases, ÝMC succeeded to manufacture an IEX material with narrow particle and pore size distribution to warrant very good chromatographic performance.
Please contact us to learn how YMC-BioPro can help in your bio-process.
From headquarters located in Kyoto, Japan, YMC has gradually and consistently matured to a level of international recognition, specifically within the pharmaceutical and biotech world, as one of the leading suppliers of analytical and process-scale silica-based stationary phases for High Performance Liquid Chromatography, preparative equipment, custom purification and method development.
New: Micro Reactor / Flow Chemistry scalable to production scale!
Qualified technical support and backup is available through operations in Japan, the United States (for the "Americas"), India, China and Germany (for Europe, Africa and the Middle East). www.ymc.de
YMC Europe GmbH
Te.: +49 (0)2064-427 270
This release was published on openPR.
Permanent link to this press release:
Please set a link in the press area of your homepage to this press release on openPR. openPR disclaims liability for any content contained in this release.
You can edit or delete your press release The Bio-Chromatographers’ wish here
News-ID: 111071 • Views: 1631
More Releases from YMC Europe GmbH
Versatile (U)HPLC-hybrid columns, now also in C8!
With YMC-TriArt challenging pH-stability and high temperatures are no longer a limitation to your work. Above all due to its unique particle composition a balanced hydrophibicty and silanol activity are achieved which makes YMC-TriArt a “First Choice” column in method development! This new, innovative stationary phase YMC-TriArt fulfils all the requirements for modern, highly demanding chromatographic separations: - Versatility: balanced hydrophobicity and silanol activity. - Flexibility:
IEX columns for material screening and method development
Are you sure your column has been packed the best possible way? Until now the extremely efficient YMC-BioPro IEX phases have only been available as bulk media. Still it was introduced into many screening procedures in industry, because of its high quality. Now it is even easier to evaluate its extremely good cost-efficiency ratio: YMC announces the introduction of pre-packed IEX columns. The columns are always perfectly packed and, after equilibration
The YMC HG-series: High Quality low price silica material
Is it possible to get the best of both worlds? Better quality at better price? It is possible with the new YMC HG-grade series of silica based materials! These new phases combine the trusted selectivity of the YMC*gel materials with a new high performance silica base. The most obvious advantage of the new silica base is its extended mechanical stability which is due to an improved production process for the base
Easy handling with Kronlab Pilot columns
Advanced service and cleaning for Kronlab glass Pilot columns As a long-time specialist in the field of glass Pilot columns for lab and production separations, Kronlab specialists are continuously in close dialogue with chromatography end-users from Pharma/Biotech as well as research and education fields. This leads to numerous technical and practical modifications which make your every-day work a lot easier. The latest innovations are the stands of the Kronlab glass Pilot columns.
More Releases for DBC
Global DBC Ceramic Substrate Market Overview Report by 2020-2025
Scope of the Report: The global DBC Ceramic Substrate market size is expected to gain market growth in the forecast period of 2020 to 2025, with a CAGR of 8.6%% in the forecast period of 2020 to 2025 and will expected to reach USD 383.8 million by 2025, from USD 275.8 million in 2019. Market segmentation DBC Ceramic Substrate market is split by Type and by Application. For the period 2015-2025, the growth
DBC Ceramic Substrate Global Market 2018 Growth Analysis Report & Forecast to 20 …
UpMarketResearch published an Exclusive report on “DBC Ceramic Substrate” delivering key insights and providing a competitive advantage to clients through a detailed report. The report contains DBC Ceramic Substrate pages which highly exhibit on current market analysis scenario, upcoming as well as future opportunities, revenue growth, pricing and profitability. This report focuses on the DBC Ceramic Substrate Market, especially in North America, Europe and Asia-Pacific, South America, Middle East and
Global Photoinitiators Market 2018 - BASF, Lambson, Arkema, DBC
Apex Market Reports, recently published a detailed market research study focused on the “Photoinitiators Market” across the global, regional and country level. The report provides 360° analysis of “Photoinitiators Market” from view of manufacturers, regions, product types and end industries. The research report analyses and provides the historical data along with current performance of the global PP Pipe industry, and estimates the future trend of Photoinitiators on the basis of
Photoinitiators market Research Report Released with growth, latest trends & for …
The latest study on the Photoinitiators Market industry includes a detailed analysis of the future trends and demands for the forecast period, 2018 - 2022. The market intelligence report on the Photoinitiators Market industry encompasses an in-depth evaluation of different products, applications, end-users and their sales across different regions. A closer look at the important industry terms and definition, technology advancements and forces influencing the product and production capability further
Global DBC Ceramic Substrate Market Comprehensive Analysis and Forecast 2018
Qyresearchreports include new market research report "Global DBC Ceramic Substrate Market Professional Survey Report 2018" to its huge collection of research reports. The market study on the global market for DBC Ceramic Substrate examines current and historical values and provides projections based on accumulated database. The report examines both key regional and domestic markets to provide a conclusive analysis about the developments in the DBC Ceramic Substrate market over the 2018-2025
Current Trends and Future Growth of Global DBC Ceramic Substrate Market
Qyresearchreports include new market research report DBC Ceramic Substrate to its huge collection of research reports. The global DBC Ceramic Substrate market has been exclusively and elaborately examined in this report while taking into account some of the most pivotal factors holding the capability to influence growth. For the said forecast tenure, the report has shed light on critical market dynamics, which include trends, opportunities, restraints, and growth drivers. The analysts