Melamine in pet-foodprotein content by addition of non-protein nitrogen and so allowing illegal dilution of the product with water with a corresponding increase in sales value.
The problem is that melamine forms crystals of melamine cyanurate from aqueous solutions when mixed with cyanuric acid (which forms naturally by hydrolysis of melamine). These low solubility, virtually indigestible crystals concentrate in the renal microtubules and form large numbers of round spoke-like, yellow crystals, damage the renal cells and ultimately kidney failure.
Recently, more and more attention has been focussed on melamine in milk and food products (including, for example, chocolate). The most distressing incident occurred in the summer of 2008 when more than 54,000 babies became seriously ill and four died in China due to suspected milk formula. However, earlier in 2007 the FDA reported that an estimated 8,500 cats and dogs died after eating melamine-contaminated pet food.
In order to avoid further accidents involving melamine contamination, it is essential to provide an analytical method, which monitors the level of melamine without compromising resolution due to excessive background signals from the sample matrix.
YMC have developed an ultra-fast HPLC method which also can be transferred to LC/MS detection and a 2 μm stationary
phase designed to analyse polar compounds, YMC-UltraHT Hydrosphere C18.
This method has been successfully developed using 2 μm Hydrosphere C18. The application allows
analysis of complex matrices in less than 2.5 minutes. The peak of interest for melamine shows
baseline separation from other components, allowing easy, fast quantification of trace contamination.
For further information please feel free to contact Dr. Friederike Becker.
From headquarters located in Kyoto, Japan, YMC has gradually and consistently matured to a level of international recognition, specifically within the pharmaceutical and biotech world, as one of the leading suppliers of analytical and process-scale silica-based stationary phases for High Performance Liquid Chromatography, preparative equipment, custom purification and method development.
New: Micro Reactor / Flow Chemistry scalable to production scale!
Qualified technical support and backup is available through operations in Japan, the United States (for the "Americas"), India, China and Germany (for Europe, Africa and the Middle East). www.ymc.de
YMC Europe GmbH
Frau Dr. Friederike Becker
Te.: +49 (0)2064-427 261
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