openPR Logo
Press release

Synbio Technologies Revolutionizes DNA Synthesis

09-04-2017 12:00 PM CET | Science & Education

Press release from: Synbio Technologies

DNA Synthesis Definition and Methods

DNA synthesis definition
DNA synthesis is defined as the process by which copies of nucleic acids are strung together to form a longer DNA sequence within a laboratory setting.

DNA synthesis processes
DNA oligomers are the foundation of the DNA synthesis process. The essential feature of DNA synthesis is that no naturally isolated DNA is used. This step deviates from the traditional method of studying genetics, by which a preexisting genetic sequence was extracted from an organism to be amplified and later studied. With gene synthesis we, at Synbio Technologies, are capable of generating de novo genetic sequences that may not currently be present anywhere within nature. Although clonal plasmid-based intermediates might exist during the assembly of a target DNA, every base originates as a phosphoramidite molecule at the beginning of DNA synthesis. Today, all DNA synthesis methods begin with solid-phase phosphoramidite chemistry to construct single-stranded DNA molecules ranging in size between 10 and 100 base pairs in length. These single stranded molecules are then enzymatically assembled into larger molecules. This process is then repeated until the required sequence length is generated. This process has been perfected by Synbio Technologies through our three phase Syno Platform. This platform offers the ability to generate sequences up to and including 200kb in length with one hundred percent accuracy, guaranteed to be identical to the sequence requested by the customer.

DNA synthesis assembly in yeast
A commonly used method of DNA fragment assembly is through the use of a yeast intermediate. The yeast, better known as Saccharomyces cerevisiae, can synthesize single-stranded DNA with a minimum length of 38 overlapping base pairs and a linear double-stranded vector in one transformation event. In this way, oligonucleotides can synthesize DNA for a wide range of lengths, ranging from as few as 20bps in length to as large as 200bps in length. DNA synthesis assembly in yeast is a useful method for building synthetic DNA molecules that are relatively large in length. Since yeast have a powerful capacity to synthesize and recombine DNA fragments, yeast has become a model eukaryote for studying numerous cellular processes. In addition to this advantage, homologous recombination in yeast can be used to build DNA fragments from overlapping constituent parts.

TopDown one-step DNA synthesis method
TopDown DNA synthesis is a simple and cost-effective method to assemble a long genetic sequences up to and including 200kb in length. The TopDown DNA synthesis method can be distinguished from conventional gene synthesis methods by two key features. First, the melting temperature of the outer primers is designed to be ~8°C lower than that of the assembly oligonucleotides. Secondly, there are different annealing temperatures that are utilized to selectively control the efficiencies of the oligonucleotide assembly and full-length template amplification. The TopDown DNA synthesis method is an effective way to remove the interference between PCR assembly and amplification. This interference between PCR assembly and amplification have been linked to the development of certain malfunctions within the DNA synthesis process. This demonstrates that TopDown DNA synthesis is a method which can optimize DNA synthesis under certain condition.

Synbio Technologies is a DNA technology company whose main focus is DNA synthesis. This is seen in our high quality products generated by Synbio Technologies’ proprietary Syno® DNA synthesis platform which can synthesize sequences up to and including 200kb in length. This length is characteristic of DNA sequences which make up genes, genomes, and various biological pathways. In addition to this, the professional Syno® Codon software can significantly optimize the original sequences from customers, and provide synthesized DNA sequences with one hundred percent accuracy within 5 days. It is these factors that have led Synbio Technologies to become one of the premier companies within the gene synthesis industry.

About Synbio Technologies:
With its Syno® technology platform as a foundation, Synbio Technologies provides efficient services for next generation recombinant antibody libraries, the synthesis of genomic chromosomes, and advanced DNA data storage capabilities.
Synbio Technologies has established a complete synthetic biology platform from the synthesis of oligonucleotides and DNA fragments to the construction of chromosome genomes. Syno®3.0, the world’s most advanced next generation of synthetic biology technology platform, provides high throughput DNA chip synthesis with exceptionally low cost. At the same time, Synbio Technologies leveraged the Syno® synthetic technology platform, to design and develop an advanced biological technology transformation and application platform, the Synbio GPS platform. In alignment with the central dogma of biology, the platform connects genotype/design and phenotype/application with the new, creation enabling, synotype. Through these “design – creation – application” technologies, Synbio Tech. enables gene discovery, and thereby advances genetic understanding and its potential to improve the individual’s lives.

Synbio Technologies
1 Deer Park Drive, Suite L-1
Monmouth Junction, NJ 08852
Logan Cummins
logan.cummins@synbio-tech.com
732-230-3003

This release was published on openPR.

Permanent link to this press release:

Copy
Please set a link in the press area of your homepage to this press release on openPR. openPR disclaims liability for any content contained in this release.

You can edit or delete your press release Synbio Technologies Revolutionizes DNA Synthesis here

News-ID: 695518 • Views: 257

More Releases from Synbio Technologies

Synbio Technologies and Large Gene Synthesis
An interesting and important aspect of genetic research that has grown drastically in popularity over the last ten to fifteen years is gene synthesis . Gene synthesis can be defined as the construction of a physical genetic sequence from a requested sequence in text format. Gene synthesis allows us, at Synbio Technologies, to manufacture genetic sequences that may not already exist within nature. In addition to the de novo sequences
Synbio Technologies and sgRNA Libraries
Synthetic DNA Library Synthesis Synthetic DNA library synthesis is widely applied mechanism within various fields of research such as: discovery biology, protein engineering, structural biology, and gene expression. All of these fields of scientific research have revolutionized the world we live in today, through various means. One of the more popular discoveries in the genetics research community was the construction of the first synthetic DNA library. A synthetic
Synbio Technologies and Next Generation DNA Synthesis
Progress in DNA Synthesis Steps Within the last few decades, advancements in methods of sequencing, as well as synthesizing, DNA sequences have improved our capabilities of understanding genomics which has led to improved capability of engineering biological systems. The chemically synthesized DNA oligonucleotides and their assembly constructs contain encoded genes, synthons, circuits, metabolic pathways and even whole genomes. These assemblies have the ability to design, build, and test various research
Synbio Tech. Announced the Establishment of Cooperative Relations with ALSTEM
Synbio Technologies Announced the Establishment of Cooperative Relations with ALSTEM Synbio Technologies, a leading international synthetic biology service company, recently announced a new partnership with ALSTEM, LLC, a United States biotech company, with the aim of developing various human CRISPR-Cas9 sgRNA libraries. These libraries will provide novel approaches for characterizing target and drug resistance genes of therapeutic significance. Related applications also promise to accelerate new drug development and advance the understanding

All 5 Releases


More Releases for DNA

Recombinant DNA Technology Market -
Recombinant DNA technology has allowed the transfer of desired characteristics which is useful to improve the existing systems by controlling expression of target genes. Recombinant DNA technology allows mass production of therapeutic proteins, which are characterized by high level of consistency and stability. The technology has multidisciplinary applications and potential to deal with important aspects of life, for instance, improving health, and enhancing food resources. The genetically modified plants have
Global DNA Vaccine Pipeline Analysis
he global market for DNA vaccines is currently recording significant growth rates owing to the lesser side effects, ease of development, lesser cost and continued development in biotechnology arena. At present, not even a single DNA vaccine has been approved for human beings. However, around 100 products are under clinical trial for treating diseases like HIV infection, cancer and other infectious diseases. With regards to animal health, there are three
Global DNA Sequencing Market Report
MarketResearchReports.Biz announces addition of new report “Global DNA Sequencing Market Report: 2015 Edition” to its database. Description DNA sequencing market is one of the fastest growing segments of the life sciences tools market. This research study includes the market assessment of various products, technologies, applications and end-users. The report briefs about the classification in genomics, process of transcription and translation of DNA, historical development, and generations of sequencing technologies. The sequencing industry is
Global DNA Sequencing Market Report
MarketResearchReports.Biz announces addition of new report “Global DNA Sequencing Market Report: 2015 Edition” to its database. Description DNA sequencing market is one of the fastest growing segments of the life sciences tools market. This research study includes the market assessment of various products, technologies, applications and end-users. The report briefs about the classification in genomics, process of transcription and translation of DNA, historical development, and generations of sequencing technologies. The sequencing industry is
DNA Chip and DNA microarray industry NextGen Frontier for DNA Technologies – I …
A report from Transparency Market Research, titled ‘DNA Chip Market - Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2015 – 2023,’ states that the global DNA chip market is expected to expand at a rapid pace during the period from 2015 to 2023 due to the increasing prevalence of cancer, the growth in personalized medicines, and technological advancements. A DNA chip, also known as a DNA microarray,
Immigration DNA test in india
Immigration Dna Test in India Advanced DNA offer Paternity, Maternity, Siblings, Immigration Dna Test etc. in India. We offer a wide range of testing methods available for establishing a biological relationship for immigration, which include both paternity, and maternity testing. However other relationships can be proved between Siblings, Aunts/Uncles, Nieces/Nephews and Grandparents through our Relationship Testing. What Is The Process I Need To Follow for an Immigration Test? Advanced DNA India our Immigration department