Comprehensive Third-Generation Sequencing Technology Market Forecast 2025-2034: Growth Trends and Strategic Shifts

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Third-Generation Sequencing Technology Market Size Valuation Forecast: What Will the Market Be Worth by 2025?
The market size of third-generation sequencing technology has seen dramatic growth in recent times. Its expansion is forecasted from $12.25 billion in 2024 to $14.00 billion in 2025, maintaining a compound annual growth rate (CAGR) of 14.2%. The surge in this historical period is due to factors such as the increasing demand for long-read sequencing, decreasing expenses related to sequencing, requirement for real-time pathogen detection, initiatives related to personalized medicine, growth observed in clinical diagnostics, and the rise of precision agriculture.

Third-Generation Sequencing Technology Market Size Forecast: What's the Projected Valuation by 2029?
Expectations are high for the third-generation sequencing technology market, with a projected surge in size over the next few years. The market is projected to reach an impressive $23.51 billion by 2029, thanks to a compound annual growth rate (CAGR) of 13.8%. Several factors will fuel this growth in the projected period including increased occurrence of prolonged and infectious diseases, enhanced applications in clinical diagnostics, the expansion into non-human genomics, a soaring increase in government and private funding, and the development of sequencing infrastructure in emerging markets. The period also expects to see key trends such as improved data analysis through AI integration, the production of real-time handheld sequencing devices, advancements in long-read sequencing precision, progress in reducing costs to allow for broader accessibility, and the progression of applications in personalized medicine.

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What Are the Drivers Transforming the Third-Generation Sequencing Technology Market?
The growth of the third-generation sequencing technology market is anticipated to surge due to the rising incorporation of personalized medicine. Personalized medicine essentially implies a medical approach directed by a person's unique genetic makeup, lifestyle, and environment. Advancements in genomics, which facilitate pinpointed identification of genetic variations and individualized treatments, predominantly drive its increasing adoption. Third-generation sequencing technology bolsters personalized medicine through the delivery of long-read, highly accurate genomic data, facilitating accurate identification of genetic variations. It paves the way for customized treatment plans by offering a thorough understanding of individual genomes, therefore, enhancing clinical results and advancing precision healthcare. As an example, in February 2024, the U.S. Food and Drug Administration (FDA) sanctioned 26 new personalized medicines, an uptake from the previous 12 approved in 2023, as reported by the Personalized Medicine Coalition (PMC), a nonprofit establishment based in the U.S. Consequently, the rising incorporation of personalized medicine fuels the prosperity of the third-generation sequencing technology market.

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What Long-Term Trends Will Define the Future of the Third-Generation Sequencing Technology Market?
Leading businesses in the third-generation sequencing technology market are concentrating their efforts on creating advanced sequencing platforms, such as highly rapid and scalable sequencing systems. These advancements aim to improve research productivity, boost clinical utility, and cut down on both the time and costs involved in the process. These ultra-rapid and scalable sequencing systems are breakthroughs in technology that provide high-volume, real-time analysis of genomes, with remarkable speed and precision, consequently aiding in exhaustive genomic profiling across several applications. For instance, Roche Holding AG, a Swiss pharmaceutical firm, introduced Sequencing by Expansion (SBX) technology in February 2025. This is a fully incorporated next-gen sequencing system which uses a patented biochemical process to convert DNA or RNA sequences into measurable surrogate polymers, referred to as Xpandomers. This facilitates highly accurate nanopore sequencing at a single-molecule level. The SBX system features ultra-fast, scalable sequencing abilities along with automated data interpretation, yielding whole-genome sequencing outcomes in merely hours instead of days, thereby eliminating the need for substantial manual input.

Which Segments in the Third-Generation Sequencing Technology Market Offer the Most Profit Potential?
The third-generation sequencing technology market covered in this report is segmented as

1) By Technology Type: Single-Molecule Real-Time Sequencing (SMRT), Transcription-Mediated Amplification (TMA), Nanopore Sequencing, Sequencing By Synthesis (SBS), Other Technology Types
2) By Workflow: Sample Preparation, Sequencing, Data Analysis, Data Interpretation, Data Storage And Management
3) By Application: Genomics, Transcriptomics, Epigenomics, Metagenomics, Personalized Medicine, Synthetic Biology
4) By End-User: Academic Institutions, Clinical Laboratories, Pharmaceutical And Biotechnology Companies, Research Organizations, Government And Non-Profit Organizations

Subsegments:
1) By Single-Molecule Real-Time Sequencing (SMRT): Zero-Mode Waveguides (ZMWs), Circular Consensus Sequencing (CCS), High-Fidelity (HiFi) Reads
2) By Transcription-Mediated Amplification (TMA): Qualitative Transcription-Mediated Amplification (TMA) Assays, Quantitative Transcription-Mediated Amplification (TMA) Assays, Multiplex Transcription-Mediated Amplification (TMA) Assays
3) By Nanopore Sequencing: Portable Nanopore Sequencers, Benchtop Nanopore Sequencers, High-Throughput Nanopore Platforms
4) By Sequencing By Synthesis (SBS): Reversible Terminator Chemistry, Real-Time Sequencing By Synthesis (SBS) Platforms, Short-Read Sequencing By Synthesis (SBS), Long-Read Sequencing By Synthesis (SBS)
5) By Other Technology Types: Hybrid Sequencing Platforms, Pyrosequencing, Ion Semiconductor Sequencing, Emerging Experimental Methods

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Which Firms Dominate the Third-Generation Sequencing Technology Market by Market Share and Revenue in 2025?
Major companies operating in the third-generation sequencing technology market are F. Hoffmann-La Roche AG, Thermo Fisher Scientific Inc., Eurofins Scientific SE, Agilent Technologies Inc., Illumina Inc., Qiagen N.V., Macrogen Inc., Myriad Genetics Inc., 10x Genomics Inc., Twist Bioscience Corporation, Takara Bio Inc., Fulgent Genetics Inc., Oxford Nanopore Technologies Limited, Ultima Genomics Inc., Element Biosciences Inc., Sophia Genetics SA, Variantyx Inc., Quantapore Inc., Phase Genomics Inc., and Singular Genomics Systems Inc.

Which Regions Offer the Highest Growth Potential in the Third-Generation Sequencing Technology Market?
North America was the largest region in the third-generation sequencing technology market in 2024. The regions covered in third-generation sequencing technology report are Asia-Pacific, Western Europe, Eastern Europe, North America, South America, Middle East and Africa.

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