Comprehensive Next-Generation Sequencing Library Preparation Market Forecast 2025-2034: Growth Trends and Strategic Shifts

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Next-Generation Sequencing Library Preparation Market Size Valuation Forecast: What Will the Market Be Worth by 2025?
The market size of next-generation sequencing library preparation has experienced a swift expansion in previous years. It is forecasted to increase from $1.42 billion in 2024 to $1.63 billion in 2025, indicating a compound annual growth rate (CAGR) of 14.7%. Factors such as the amplifying usage in monitoring infectious diseases, escalating funding towards genomic research, enhanced utility amid the pandemic, better access for research laboratories, and its expanding role in precision medicine have contributed to the growth in the historic period.

Next-Generation Sequencing Library Preparation Market Size Forecast: What's the Projected Valuation by 2029?
The market for next-generation sequencing library preparation is set to expand significantly in the upcoming years, reaching a value of $2.78 billion in 2029 with a compound annual growth rate (CAGR) of 14.3%. This surge during the prediction period is due to the increasing use in clinical diagnostics, the escalating demand for personalized medications, growing initiatives in oncology research, a greater requirement for high-throughput sequencing, and a stronger emphasis on reducing the time needed to produce results. Salient trends during this forecasting period encompass improvements in workflow efficiency, incorporation of artificial intelligence into data analysis, standardization of protocols facilitated by technology, enhancements in user-friendly systems, and advancements in cloud-based data solution.

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What Are the Drivers Transforming the Next-Generation Sequencing Library Preparation Market?
The expansion of the next-generation sequencing library preparation market is predicted to be fuelled by the increasing interest in gene therapy. This approach to treating illness involves modifying a person's genes to prevent or cure diseases. The importance of gene therapy is growing as a result of the increasing number of genetic disorders that necessitate long-term, targeted treatment strategies. The preparation of high-quality DNA or RNA samples for sequencing, a process facilitated by next-generation sequencing (NGS) library preparation, is essential in the accurate analysis and validation of genetic modifications in gene therapy. As an illustration, the American Society of Gene and Cell Therapy reported a 10% increase in phase III gene therapy clinical trials in April 2024, making it the first quarterly rise since the third quarter of 2022. This uptick in gene therapy ambition is hence propelling the growth of the next-generation sequencing library preparation market.

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Which Emerging Trends Are Transforming the Next-Generation Sequencing Library Preparation Market in 2025?
Leading firms in the next-generation sequencing library preparation market are concentrating on the creation of sophisticated advancements, such as Xpandomer technology. This technology enhances the pace of sequencing, augments the precision of data, and escalates scalability. This approach allows for more streamlined operations, thus enabling more robust and efficient genomic examination. Xpandomer technology is a sophisticated DNA or RNA sequencing technique that transforms a targeted nucleic acid molecule into a synthetic, expanded polymer known as an Xpandomer. This is 50 times longer than the original DNA or RNA strand. For example, in February 2025, Roche AG, a biotechnology corporation based in Switzerland, introduced Sequencing by Expansion, an advanced sequencing platform crafted to enhance genomic resolution for superior diagnostics, disease research, and precision medicine applications. This pioneering automated next-generation sequencing (NGS) library preparation mechanism simplifies intricate operations, enriches data quality, and boosts genomic examination to support advanced study and personalized medication. The sequencing by expansion platform is incredibly flexible, facilitating precise evaluation of varied genomic changes across a broad scope of diseases, such as cancer and uncommon genetic disorders, by improving resolution and accuracy for in-depth biological comprehension.

Which Segments in the Next-Generation Sequencing Library Preparation Market Offer the Most Profit Potential?
The next-generation sequencing library preparation market covered in this report is segmented

1) By Sequencing Type: Targeted Genome Sequencing, Whole Genome Sequencing, Whole Exome Sequencing, Other Sequencing Types
2) By Product: Reagents And Consumables, DNA Library Preparation Kits, Library Preparation Kits, RNA Library Preparation Kits, Other Products
3) By Technology: Ion Semiconductor Sequencing, Sequencing By Synthesis (SBS), Nanopore Sequencing, Single-Molecule Real-Time Sequencing (SMRT), Other Technologies
4) By Application: Drug And Biomarker Discovery, Disease Diagnostics, Cancer Diagnostics, Reproductive Health Diagnostics, Infectious Disease Diagnostics, Other Applications
5) By End-User: Hospitals And Clinics, Academic And Research Institutions, Pharmaceutical And Biotechnology Companies, Other End Users

Subsegments:
1) By Targeted Genome Sequencing: Amplicon Sequencing, Hybridization Capture-Based Sequencing, CRISPR-Based Target Enrichment, Multiplex PCR
2) By Whole Genome Sequencing: Human Whole Genome Sequencing, Animal Whole Genome Sequencing, Plant Whole Genome Sequencing, Microbial Whole Genome Sequencing
3) By Whole Exome Sequencing: Clinical Whole Exome Sequencing, Research Whole Exome Sequencing, Custom Exome Sequencing, Population-Scale Exome Sequencing
4) By Other Sequencing Types: RNA Sequencing, ChIP Sequencing (ChIP-Seq), Methylation Sequencing, Single-Cell Sequencing

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Which Firms Dominate the Next-Generation Sequencing Library Preparation Market by Market Share and Revenue in 2025?
Major companies operating in the next-generation sequencing library preparation market are F. Hoffmann-La Roche Ltd., Thermo Fisher Scientific Inc., Agilent Technologies India Private Limited, Illumina Inc., Beckman Coulter Inc., Bio-Rad Laboratories Inc., IDT Corporation, Tecan Group AG, Hamilton Company, Takara Bio Inc., 10x Genomics Inc., Oxford Nanopore Technologies Plc, Twist Bioscience Corporation, New England Biolabs Inc., SPT Labtech Limited, Watchmaker Genomics Inc., Pillar Biosciences Inc., Aurora Biomed Inc., BGI Group, and SEQWELL INC.

Which Regions Offer the Highest Growth Potential in the Next-Generation Sequencing Library Preparation Market?
North America was the largest region in the next-generation sequencing library preparation market in 2024. Asia-Pacific is expected to be the fastest-growing region in the forecast period. The regions covered in the next-generation sequencing library preparation market report are Asia-Pacific, Western Europe, Eastern Europe, North America, South America, Middle East, Africa.

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