Automated Next-Generation Sequencing (NGS) Library Preparation Market Growth Accelerates: Strategic Forecast Predicts $1.20 Billion by 2029

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What Will the Automated Next-Generation Sequencing (NGS) Library Preparation Industry Market Size Be by 2025?
The market size for automated next-generation sequencing (NGS) library preparation has been on a fast-paced expansion over the recent years. The projection reveals an increase from $0.63 billion in 2024 to $0.72 billion in 2025, representing a compound annual growth rate (CAGR) of 13.8%. This remarkable growth during the historic period reflects a rise in its usage in monitoring infectious diseases, increased funding for genomic studies, broadened usage in times of pandemics, enhanced availability for research laboratories, and a surge in its application in the field of precision medicine.

What's the Long-Term Growth Forecast for the Automated Next-Generation Sequencing (NGS) Library Preparation Market Size Through 2029?
Over the next few years, the market size of automated next-generation sequencing (NGS) library preparation is anticipated to witness significant growth. The market is predicted to expand to $1.20 billion by 2029, with a compound annual growth rate (CAGR) of 13.4%. The upswing projected in the forecast period can be credited to the escalating application in clinical diagnostics, surging demand for customized medicine, expansion of uses in oncology research, the rising necessity for high-throughput sequencing, and the increasing emphasis on minimizing turnaround time. During the forecast period, key trends include improvements in workflow efficiency, the inclusion of artificial intelligence in data interpretation, standardization of practices powered by technology, advancements in user-friendly systems, and progress in cloud-based data solutions.

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What Are the Key Growth Drivers Fueling the Automated Next-Generation Sequencing (NGS) Library Preparation Market Expansion?
The surge in gene therapy is anticipated to foster the expansion of the automated next-generation sequencing (NGS) library preparation market going forward. Gene therapy is a therapeutic approach that modifies an individual's genes to cure or ward off diseases. The growth of gene therapy can be attributed to the increasing incidence of genetic disorders that necessitate precise and enduring treatment solutions. Automated NGS library preparation is employed in gene therapy to precisely analyze and verify genetic alterations by creating superior quality DNA or RNA samples for sequencing. For example, in April 2024, the American Society of Gene and Cell Therapy, a U.S. based non-profit medical and scientific entity, reported a 10% rise in the number of stage III gene therapy clinical trials in the fourth quarter, marking the initial quarterly rise since the third quarter of 2022. Therefore, the surge in gene therapy is propelling the expansion of the automated next-generation sequencing (NGS) library preparation market.

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What Trends Will Shape the Automated Next-Generation Sequencing (NGS) Library Preparation Market Through 2029 and Beyond?
Leading businesses in the automated next-generation sequencing (NGS) library preparation market are concentrating on the creation of state-of-the-art technologies, like Xpandomer technology, that optimize sequencing speed, enhance data precision, and amplify scalability. This technology consequently simplifies operations and facilitates more effective and dependable genomic assessments. Xpandomer technology is a revolutionary technique of sequencing deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), where the targeted nucleic acid molecule is converted into a synthetic, expanded polymer called an Xpandomer, which is roughly 50 times larger than the initial DNA or RNA strand. For example, in February 2025, a biotech firm based in Switzerland, Roche AG, introduced Sequencing by Expansion, a pioneering sequencing platform designed to amplify genomic resolution for disease research, advanced diagnostics, and precision medicine applications. This novel automated next-generation sequencing (NGS) library preparation system simplifies intricate workflows, boosts data quality, and hastens genomic analysis to aid advanced research and personalized medicine. The platform is highly adaptable, providing exact analysis of several genomic alterations across a plethora of diseases, such as cancer and rare genetic conditions, by improving resolution and precision for a deeper comprehension of biological aspects.

How Is the Automated Next-Generation Sequencing (NGS) Library Preparation Market Segmented?
The automated next-generation sequencing (NGS) library preparation market covered in this report is segmented

1) By Type: Targeted Genome Sequencing, Whole Genome Sequencing, Whole Exome Sequencing, Other Sequencing Types
2) By Workflow: Nucleic Acid Extraction, Fragmentation, Adapter Ligation, Purification And Quantification
3) By Application: Drug And Biomarker Discovery, Disease Diagnostics, Other Applications
4) By End-Use: Hospitals And Clinics, Academic And Research Institutions, Pharmaceutical And Biotechnology Companies, Other End-Users

Subsegments:
1) By Targeted Genome Sequencing: Amplicon-Based Targeted Sequencing, Hybridization Capture-Based Targeted Sequencing, Custom Targeted Panels, Pre-designed Gene Panels
2) By Whole Genome Sequencing: Human Whole Genome Sequencing, Animal Whole Genome Sequencing, Plant Whole Genome Sequencing, Microbial Whole Genome Sequencing
3) By Whole Exome Sequencing: Standard Exome Sequencing, Clinical Exome Sequencing, Custom Exome Panels, Ultra-Rapid Exome Sequencing
4) By Other Sequencing Types: RNA Sequencing (RNA-Seq), Epigenome Sequencing, Metagenomic Sequencing, Single-Cell Sequencing

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Which Companies Are Leading the Charge in Automated Next-Generation Sequencing (NGS) Library Preparation Market Innovation?
Major companies operating in the automated next-generation sequencing (NGS) library preparation market are F. Hoffmann-La Roche Ltd., Thermo Fisher Scientific Inc., Agilent Technologies India Private Limited, Illumina Inc., Beckman Coulter Inc., Bio-Rad Laboratories Inc., IDT Corporation, Tecan Group AG, Hamilton Company, Takara Bio Inc., 10x Genomics Inc., Oxford Nanopore Technologies plc, Twist Bioscience Corporation, New England Biolabs Inc., SPT Labtech Limited, Watchmaker Genomics Inc., Pillar Biosciences Inc., Aurora Biomed Inc., BGI Group, SEQWELL INC., and Hudson Robotics Inc.

Which Regions Are Leading the Global Automated Next-Generation Sequencing (NGS) Library Preparation Market in Revenue?
North America was the largest region in the automated next-generation sequencing (NGS) library preparation market in 2024. Asia-Pacific is expected to be the fastest-growing region in the forecast period. The regions covered in the automated next-generation sequencing (NGS) library preparation market report are Asia-Pacific, Western Europe, Eastern Europe, North America, South America, Middle East, Africa.

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