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Real-time PCR (TaqMan) Primer and Probes Design Tool

04-28-2023 02:29 PM CET | Health & Medicine

Press release from: GenScript

Real-time PCR (TaqMan) Primer and Probes Design Tool

Real-time PCR (TaqMan) Primer and Probes Design Tool

Real-time PCR (polymerase chain reaction) is an essential molecular biology technique that enables the quantification and amplification of specific DNA or RNA sequences in real-time. TaqMan is a widely used real-time PCR method that employs fluorescent probes to detect and quantify DNA or RNA targets. In this article, we will discuss the significance of real-time PCR and the design of TaqMan primers and probes using an online tool available at GenScript.

Real-time PCR has a multitude of advantages over conventional PCR techniques, such as greater sensitivity, specificity, accuracy, and rapid results. The technique can detect and quantify DNA or RNA targets present in a sample as low as a few copies, making it a valuable tool in applications ranging from clinical diagnostics to genetic research. Real-time PCR is also quantitative, allowing for the accurate determination of the amount of DNA or RNA in a sample.

The TaqMan assay is a popular real-time PCR method that uses fluorescent probes to detect and quantify DNA or RNA targets. The TaqMan probes are designed to anneal to the target DNA or RNA sequence and comprise a fluorescent reporter dye and a quencher molecule. During PCR, the Taq polymerase cleaves the probe, causing the reporter dye to separate from the quencher molecule, leading to an increase in fluorescence. The amount of fluorescence produced is proportional to the amount of target DNA or RNA in the sample.

Designing TaqMan primers and probes can be a complex and challenging task that requires a deep understanding of the target sequence and probe design principles. GenScript online TaqMan primer and probe design tool employs sophisticated algorithms that take into account various parameters, such as melting temperature, specificity, and secondary structure, to design highly specific and efficient TaqMan primers and probes. The user can input the desired target sequence and parameters such as product size, Tm range, and exclusion regions to generate optimized primers and probes.

Using GenScript TaqMan primer and probe design tool is simple and straightforward. The user can input the target sequence and select parameters, such as the Tm range, amplicon size, and exclusion regions, and the tool will generate optimized primers and probes that meet the specified criteria. The tool also provides additional information, such as melting temperature, GC content, and the presence of hairpin loops or secondary structures, to ensure the accuracy and specificity of the designed primers and probes.

Real-time PCR is a powerful molecular biology technique used in various fields, and the TaqMan assay is a popular real-time PCR method that employs fluorescent probes to detect and quantify DNA or RNA targets. GenScript TaqMan primer and probe design tool (https://www.genscript.com/tools/real-time-pcr-taqman-primer-design-tool
) is a valuable resource for designing highly specific and efficient primers and probes for real-time PCR assays. The tool's sophisticated algorithms and user-friendly interface make it an ideal tool for researchers seeking optimized TaqMan primers and probes for their experiments.

Company name: GenScript Biotech
Address: 860 Centennial Ave. Piscataway NJ 08854 united states
Contact: +1-732-885-9188
Contact: website@genscript.com

GenScript Biotech Corporation (HK.1548) is the world's leading technology and service provider of life science R&D and manufacture. Built upon its solid gene synthesis technology, GenScript Biotech is divided into four major platforms including the life science service and product platform, the biologics contract development and manufacturing organization (CDMO) platform, the global cell therapy platform and the industrial synthesis biological product platform.

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