MobiSpin S-400 – Recommended by the BfR for the Detection of Norovirus in Food

MobiSpin S-400 columns were integrated by the BfR in an optimized procedure for Norovirus detection in frozen raspberries. The additional purification of virus RNA with the columns leads to clearly enhanced detection rates by RT-PCR.

The Federal Institute for Risk Assessment (BfR) is a federal legal institution of public law of the Federal Republic of Germany. The institute is assigned to the business division of the Federal Ministry of Food and Agriculture (BMEL) and has the task of providing scientific advice to the Federal Government on questions of food safety, product safety, contaminants in the food chain, animal welfare, and consumer health protection.

In a recent paper the BfR reported on comparison and optimization of detection methods for noroviruses in frozen strawberries containing different amounts of RT-PCR inhibitors.

A paramount aspect of the work was the assessment of means aimed at the removal of RT-PCR-inhibiting substances. The authors conclude:

"The MobiSpin S-400 columns contain a Sephacryl®-based matrix (MoBiTec GmbH). It can be used in a quick and easy centrifugation procedure. During the purification process, the sample interacts with the Sephacryl®-based matrix leading to a binding of small molecules, whereas larger molecules are able to pass the column. Most of the RT-PCR-inhibiting substances are small molecules, which should therefore be removed by this purification step, whereas the larger RNA molecules should not be affected by the procedure. Indeed, the results of our experiments show that the NoV RRs could be improved for the batches 3 and 4, which were considered to contain high amounts of RT-PCR inhibitors."

The described MobiSpin columns are designed for a wide variety of nucleic acid purification applications.

Features

• Compatible with laboratory standard
• Column comes pre-packed and equilibrated
• Easy handling: spin, load sample, spin and collect the purified product
• No sample dilution
• Reproducible results with simplified protocols
• One sample in less than 4 minutes
• Numerous samples can be processed simultaneously
• Large number of applications

Applications

• Buffer exchange between enzyme reactions
• DNA purification prior to sequencing
• Oligonucleotide purification after synthesis
• Removal of free nucleotides
• Low cost plasmid purification

For details see:
https://www.mobitec.com/cms/products/bio/06_dna_prot_tools/extract_prod6.html

About MoBiTec GmbH

MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.

In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.

MoBiTec GmbH, Lotzestr. 22a,
37083 Goettingen, Germany
info@mobitec.com
www.mobitec.com

This release was published on openPR.